Scanning Switch-off Microscopy for Super-Resolution Fluorescence Imaging
Super-resolution (SR) microscopy provides a revolutionary optical imaging approach by breaking the diffraction limit of light, while the commonly required special instrumentation with complex optical setup hampers its popularity. Here, we present a scanning switch-off microscopy (SSM) concept that exploits the omnipresent switch-off response of fluorophores to enable super-resolution imaging using a commercial confocal microscope. We validated the SSM model with theoretical calculations and experiments. An imaging resolution of ∼100 nm was obtained for DNA origami nanostructures and cellular cytoskeletons using fluorescent labels of Alexa 405, Alexa 488, Cy3, and Atto 488. Notably, super-resolution imaging of live cells was realized with SSM, by employing a dronpa fluorescent protein as the fluorescent label. In principle, this SSM method can be applied to any excitation laser scanning-based microscope.
Authors
- Chunhai Fan (ORCID: https://orcid.org/0000-0002-7171-7338)
- Zhaoshuai Gao (ORCID: https://orcid.org/0009-0000-4259-9953)
- Suhui Deng (ORCID: https://orcid.org/0000-0003-3527-6093)
- Shangguo Hou (ORCID: https://orcid.org/0000-0001-6394-6467)
- Le Liang (ORCID: https://orcid.org/0000-0001-6682-959X)
- Bin Kang (ORCID: https://orcid.org/0000-0002-8304-103X)
- Weina Fang (ORCID: https://orcid.org/0000-0002-5118-4853)
- Qian Li (ORCID: https://orcid.org/0000-0002-1166-6583)
- Fei Wang (ORCID: https://orcid.org/0000-0003-3948-6899)
- Linjie Guo (ORCID: https://orcid.org/0009-0005-1159-3280)
- Hong‐Yuan Chen (ORCID: https://orcid.org/0000-0001-5217-668X)
Institutions
- Nanchang University (CN)
- Shanghai Jiao Tong University (CN)
- Chinese Academy of Sciences (CN)
- Wuhan University (CN)
- Shenzhen Bay Laboratory (CN)
- Shanghai Institute of Applied Physics (CN)
- Nanjing University (CN)
Publication Details
- Journal
- Nano Letters
- Published
- 2024-09-19
- DOI
- https://doi.org/10.1021/acs.nanolett.4c02452
- Citations
- 6
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 3.97
Funders
- National Natural Science Foundation of China
- Science and Technology Commission of Shanghai Municipality
- Nanchang University
- National Key Research and Development Program of China