Duck hepatitis A virus utilizes PCBP2 to facilitate viral translation and replication
Duck hepatitis A virus type 1 (DHAV-1) is an important member of the Picornaviridae family that causes highly fatal hepatitis in ducklings. Since picornaviruses have small genomes with limited coding capacity, they must utilize host proteins for viral cap-independent translation and RNA replication. Here, we report the role of duck poly(rC)-binding protein 2 (PCBP2) in regulating the replication and translation of DHAV-1. During DHAV-1 infection, PCBP2 expression was upregulated. A biotinylated RNA pull-down assay revealed that PCBP2 positively regulates DHAV-1 translation through specific interactions with structural domains II and III of the DHAV-1 internal ribosome entry site (IRES). Further studies revealed that PCBP2 promotes DHAV-1 replication via an interaction of its KH1 domain (aa 1-92) with DHAV-1 3D<sup>pol</sup>. Thus, our studies demonstrated the specific role of PCBP2 in regulating DHAV-1 translation and replication, revealing a novel mechanism by which host‒virus interactions regulate viral translation and replication. These findings contribute to further understanding of the pathogenesis of picornavirus infections.
Authors
- Qiao Yang (ORCID: https://orcid.org/0000-0001-7401-2168)
- Mafeng Liu (ORCID: https://orcid.org/0000-0002-6834-3128)
- Shaqiu Zhang
- Xumin Ou (ORCID: https://orcid.org/0000-0003-0456-6362)
- Anchun Cheng (ORCID: https://orcid.org/0000-0001-6093-353X)
- Renyong Jia (ORCID: https://orcid.org/0000-0002-3539-9184)
- Dekang Zhu (ORCID: https://orcid.org/0000-0002-7314-1088)
- Chenxia Xu (ORCID: https://orcid.org/0000-0003-4593-3202)
- Wei Zhang (ORCID: https://orcid.org/0000-0002-3378-1451)
- Juan Huang (ORCID: https://orcid.org/0000-0003-3072-4352)
- Shun Chen (ORCID: https://orcid.org/0000-0002-7488-1037)
- Yu He (ORCID: https://orcid.org/0000-0003-3992-0966)
- Bin Tian
- Di Sun
- Ying Wu
- Mingshu Wang
- Zhen Wu
- Yurui Jiang
- Xinxin Zhao
Institutions
- Ministry of Education of the People's Republic of China (CN)
- Sichuan Agricultural University (CN)
- China Animal Disease Control Center (CN)
- China National Pharmaceutical Group Corporation (China) (CN)
- Sichuan Center for Disease Control and Prevention (CN)
Publication Details
- Journal
- Veterinary Research
- Published
- 2024-09-19
- DOI
- https://doi.org/10.1186/s13567-024-01369-9
- Citations
- 2
- Primary Topic
- Viral Infections and Immunology Research
- Type
- article
- Field-Weighted Citation Impact
- 0.80
Funders
- China Agricultural Research System
- Sichuan Veterinary Medicine and Drug Innovation Group of China Agricultural Research System