Green synthesis, characterization, antioxidant, anti-fungal, antibacterial activities of a new 1-isopropyl-3,5-di-2-tolyl-1,3,5-triazacyclohexane

This work presents the green synthesis of a novel of 1- isopropyl- 3, 5- di-2-tolyl- 1, 3, 5-triazacyclohexane in good yield. It is made without a solvent, energy, and a catalyst as a green technique via a one-pot multi component condensation reaction between one equivalent of isopropylamine, two equivalents of o-toluidine, and three equivalents of formaline. The compound's structure has been determined using several spectroscopic methods, including thin-layer chromatography (TLC), ultraviolet-visible spectroscopy (UV-Vis), infrared spectroscopy (IR), and nuclear magnetic resonance spectroscopy (NMR) for both 1H and 13C nuclei. Additionally, the compound 'smelting point was determined. Subsequently, the molecule was evaluated for its antioxidant and biological activities. The antioxidant activity exhibited good performance in both the ABTS and Phenanthrolinetests. The antimicrobial activity of this novel compound was tested against four bacterialstrains, including two Gram-positive bacteria (Staphylococcus aurous ATCC 25923 and Bacillus cereus ATCC 10876) and two Gram-negative bacteria (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853), as well as two fungal strains (Fusarium oxysporum and Alternariaalternata)using the agar diffusion method and the direct contact method, respectively. The obtained results exhibited a high level of antibacterial and antifungal activity.