DNA Barcoding of the Endemic Freshwater Catfish Encheloclarias tapeinopterus (Bleeker, 1853) (Siluriformes:Clariidae) from Bangka Island, Indonesia

Abstract Encheloclarias tapeinopterus , an endemic catfish exclusively found in Bangka Island, Indonesia. The population of this species has been observed to be decreasing at an alarming rate, which has resulted in the IUCN Red List classifying it as a species that is vulnerable (VU). During our expedition on the 25th and 26th of January 2023, a total of Two specimens of E. tapeinopterus were collected from Kelumpik River, Pinang Sebatang Village, Central Bangka Regency, Bangka Island, Indonesia. In this groundbreaking study, we proudly present the pioneering documentation of E. tapeinopterus DNA barcoding, focusing on the Cytochrome C oxidase Subunit I (COI) gene. The recorded DNA Barcode of E. tapeinopterus has been officially registered in the Genbank under the accession code OQ281707. By employing DNA barcoding, we offer an advanced and expedient method for precise species identification and discovery, revolutionizing the field of taxonomy. Phylogenetic analysis revealed that E. tapeinopterus forms a distinct clade alongside the Clarias and Heterobranchus genus, shedding light on its evolutionary relationships and genetic affinities.