Smartphone-enhanced nanozyme sensors: Colorimetric and fluorescence sensing techniques

The advent of smartphone technology has changed the biosensing field, especially by integrating nanozyme-based colorimetric, fluorescence sensing strategy. This review article describes the development of smartphone-assisted nanozyme sensor technology, harboring increased sensitivity and specificity for detecting relevant markers. While mimicking natural catalytic enzymes, nanozymes show exceptional performance in a variety of analytical applications. Combining the computing power and imaging capabilities of smartphones, nanozyme based sensors offer a low-cost, user-friendly, and scalable solution for point-of-care technology. We have discussed the principles behind colorimetric and fluorescence sensing techniques, the role of nanozymes in these processes, and the methodologies for leveraging smartphone technology for data acquisition and analysis. Crucial applications in medical diagnostics, environmental monitoring, and food safety are reviewed, with a focus on recent innovations and forthcoming prospects. The convergence of nanozyme sensors and smartphone technology promises to democratize access to advanced colorimetric or fluorometric based biosensing. • Integration of nanozymes with smartphones for colorimetric or fluorescence-based point-of-care devices. • Smartphone-based colorimetric or fluorometric sensing with nanozymes in food, biomedical, and environmental applications. • Nanozyme reactions producing colorimetric and fluorometric signals. • Reports from 2018 to 2024 on nanozyme plus smartphone-assisted colorimetric and fluorescence-based sensing.