Impact of prematurity on LINE-1 promoter methylation
<b>Aim:</b> Promoter methylation of LINE-1 may be affected by prematurity, but there is little evidence in the literature.<b>Materials & methods:</b> Blood from premature and full-term neonates on days 0, 5, 30 and 90 was analyzed for DNA methylation percentage in a promoter region of the LINE-1, after bisulfite conversion and pyrosequencing.<b>Results:</b> Premature infants, as a whole, showed significantly lower methylation percentage at birth, but this difference diminished over time. However, the subgroup of extremely premature (<28 weeks gestational age) had higher methylation percentages, similar to full-term newborns.<b>Conclusion:</b> This research underscores the critical role of prematurity on the methylation pattern of LINE-1. These findings underline the complexity of epigenetic regulation in prematurity and emphasize the need for further studies.
Authors
- Aline de Araújo Brasil (ORCID: https://orcid.org/0000-0002-0890-5395)
- Leo Travassos Vieira Milone (ORCID: https://orcid.org/0009-0003-4666-3520)
- Antônio José Lêdo Alves da Cunha (ORCID: https://orcid.org/0000-0003-3592-1849)
- Fernanda Lima‐Setta (ORCID: https://orcid.org/0000-0001-9171-2043)
- Paulo Victor Barbosa Eleutério dos Santos (ORCID: https://orcid.org/0000-0002-4936-2120)
- Arnaldo Prata‐Barbosa (ORCID: https://orcid.org/0000-0002-4726-9782)
- Jaqueline Rodrigues Robaina (ORCID: https://orcid.org/0000-0003-1483-9029)
- Maria Clara de Magalhães‐Barbosa (ORCID: https://orcid.org/0000-0003-0959-0775)
- Gustavo Rodrigues-Santos (ORCID: https://orcid.org/0000-0001-7947-8207)
- Margarida dos Santos Salú (ORCID: https://orcid.org/0000-0002-4529-5629)
- Geórgia Chalfun (ORCID: https://orcid.org/0000-0002-8074-3503)
- Mariana Barros Genuíno de Oliveira (ORCID: https://orcid.org/0000-0002-2734-8205)
- Stephanie Cristina Alves de Oliveira Saide (ORCID: https://orcid.org/0009-0001-5732-0377)
Institutions
- Universidade Federal do Rio de Janeiro (BR)
- Maternity Foundation (DK)
- D’Or Institute for Research and Education (BR)
- Institut Privé de Gestion Institut Supérieur de Technologie Industrielle (SN)
Publication Details
- Journal
- Epigenomics
- Published
- 2024-09-16
- DOI
- https://doi.org/10.1080/17501911.2024.2397329
- Primary Topic
- Immune responses and vaccinations
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Instituto D'Or de Pesquisa e Ensino
- Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
- Universidade Federal do Rio de Janeiro