A Dicyanoisophorone-based Fluorescent Turn-on Probe for Rapid Detecting Thiophenol in Aqueous Medium and Living Cell Imaging
A novel colorimetric and fluorescent thiophenol probe based on dicyanoisophorone has been successfully achieved, which has low-cost, easy operation, high selectivity, sensitivity and stability. The chemosensor shows a large Stokes shift and approximately 170 nm when excited at 510 nm. ISO-DiNO<sub>2</sub> could be utilized as "Turn-on" and a naked-eyes chemosensor to detect PhSH, which is accompanied by a distinct color shift from red to dark purple with strong red fluorescence at 365 nm UV-light. Its limit of detection was determined to be 1.15 μM. More importantly, ISO-DiNO<sub>2</sub> can react instantaneously (<10 s) with PhS<sup>-</sup>. In addition, ISO-DiNO<sub>2</sub> has been utilized in test paper strips, water sample together with imaging of PhS<sup>-</sup> in living Raw264.7 cells, demonstrating that ISO-DiNO<sub>2</sub> has excellent and promising applications.
Authors
- Zhaoli Xue (ORCID: https://orcid.org/0000-0001-7149-7374)
- Yaqiao Shi (ORCID: https://orcid.org/0000-0001-7809-0059)
- Yue Wang (ORCID: https://orcid.org/0000-0002-7022-518X)
- Liting Jiang (ORCID: https://orcid.org/0000-0002-2342-2017)
- Jianwei Wu (ORCID: https://orcid.org/0000-0002-8137-2687)
- Ru Feng (ORCID: https://orcid.org/0009-0009-7080-1712)
- Haoxiang Bai
Institutions
- Jiangsu University (CN)
Publication Details
- Journal
- Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy
- Published
- 2024-09-20
- DOI
- https://doi.org/10.1016/j.saa.2024.125180
- Citations
- 5
- Primary Topic
- Sulfur Compounds in Biology
- Type
- article
- Field-Weighted Citation Impact
- 1.47
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Jiangsu Province