Features of the immune profile of workers of a non-ferrous metallurgy enterprise in conditions of contamination of biological media with rare earth elements (using the example of thulium)

Introduction. The active use of rare earth elements, including thulium (Tm), in various technological processes increases the risks of health problems associated with the professional activities of the employee. The purpose of the study is to study the characteristics of the immune profile of workers at a non-ferrous metallurgy enterprise under conditions of contamination of biological media with rare earth elements (using the example of thulium). Materials and methods. 35 employees of a non-ferrous metallurgy enterprise were examined. The comparison group consisted of workers with the level of thulium concentration in the blood within the reference interval (n=17), the observation group — workers whose blood thulium content was 2 times higher than the upper limit of the reference values (n=18). The content of thulium in biological media (blood) was studied using mass spectrometry with inductively coupled plasma. Flow cytometry was used to detect Annexin V-FITC+7AAD+-lymphocytes (late apoptosis/necrosis), enzyme immunoassay — IL6, TNF, general IgE, allergosorbent — specific IgG to lanthanides. Results. It was found that the workers in the observation group had a 2.3-fold increase in the content of total IgE and a 2.0-fold increase in the expression of specific IgG to lanthanides in relation to the results of the comparison group (p<0.05). It was found that with an excess thulium content in the blood, there is an inhibition of cell death by 15% relative to the values of the comparison group. Limitations of the study. Limitations of the study relate to the limited sample size. Ethics. The study protocol was approved by the Biomedical Ethics Committee of the local ethical committee of the Federal Budgetary Institution "FSC MPT URZN" No. 2 dated March 22, 2023. The study was carried out in accordance with the requirements set out in the WMA Declaration of Helsinki "Ethical Principles for Medical Research Involving Human Subjects" (1964, 2013). Voluntary informed consent to participate in the study and use of personal data was signed by all participants.