Progress in the Application of CRISPR/Cas Family Mediated Third-generation Sequencing Technology

The first generation sequencing technology based on the dideoxynucleotide (ddNTP) chain termination method proposed by Sanger was gradually eliminated due to its high cost, low sequencing read length and cumbersome process. The second generation of sequencing technology, called High-throughput sequencing (HTS), which was then developed, still has the problem of fixed read length. In recent years, the third generation sequencing technology represented by SMRT technology and Nanopore sequencing technology has gradually become popular. Compared with the previous two generations of sequencing technology, the most significant advantage of the third generation sequencing technology is its ability to carry out single molecule sequencing. In this process, the infinite length of nucleic acid sequence can be determined theoretically without the help of PCR amplification. This paper first introduces the basic principles, advantages and disadvantages of third-generation sequencing, and introduces in detail the CRISPR/Cas family-mediated SMRT technology and Nanopore sequencing technology in the third-generation sequencing technology. Finally, the research progress and prospects of the combination of the third generation sequencing technology and gene editing technology in the future are analyzed.