Development and characterization of segment-specific enteroids from the pig small intestine in Matrigel and transwell inserts: insights into susceptibility to porcine epidemic diarrhea Virus
This segment-specific enteroid culture model provides a reliable platform for virological studies, offering a controlled environment that overcomes the limitations of <i>in vivo</i> and traditional cell culture methods. Standardizing culture conditions and characterizing the model are essential for advancing enteroid-based infection models.
Authors
- Gino Castillo (ORCID: https://orcid.org/0000-0001-5997-5206)
- Lu Yen (ORCID: https://orcid.org/0000-0001-9300-9191)
- Luis G. Giménez‐Lirola (ORCID: https://orcid.org/0000-0002-4407-7996)
- Juan Carlos Mora-Díaz (ORCID: https://orcid.org/0000-0002-0725-122X)
- Ning-Chieh Twu
- Panchan Sitthicharoenchai (ORCID: https://orcid.org/0000-0002-4412-7121)
- Rahul K. Nelli (ORCID: https://orcid.org/0000-0001-8765-0943)
Institutions
- North Carolina State University (US)
- Iowa State University (US)
- Universidad Peruana Cayetano Heredia (PE)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2024-09-17
- DOI
- https://doi.org/10.3389/fimmu.2024.1451154
- Citations
- 3
- Primary Topic
- Animal Virus Infections Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.02
Funders
- Iowa State University