Co-expression of endoglucanase and cellobiohydrolase from yak rumen in lactic acid bacteria and its preliminary application in whole-plant corn silage fermentation
Consequently, the recombinant strains <i>L. lactis-cbh</i>, <i>L. lactis-eg</i>, and <i>L. lactis-eg-cbh</i> were successfully constructed, which could successfully expressed EG, CBH, and EG-CBH. <i>L. lactis-eg</i> promoted silage fermentation by degrading cellulose to produce sugar, enabling the secretory expression of EG, CBH, and EG-CBH for potential industrial applications in cellulose degradation.
Authors
- Yongjie Sunkang (ORCID: https://orcid.org/0000-0002-1701-9598)
- Xuerui Wan
- Huitian Gou
- Yaqin Wei (ORCID: https://orcid.org/0000-0001-6553-751X)
- Zhao Zhang (ORCID: https://orcid.org/0000-0001-9345-3663)
- Yijun Chen (ORCID: https://orcid.org/0000-0003-3139-8958)
- Chuan Wang (ORCID: https://orcid.org/0000-0001-5143-1714)
- Yu Xue (ORCID: https://orcid.org/0000-0002-9883-9722)
- Yuze Yang
Institutions
- Shanxi Agricultural University (CN)
- Gansu Agricultural University (CN)
- Gansu Academy of Sciences (CN)
- National Animal Husbandry Service (CN)
Publication Details
- Journal
- Frontiers in Microbiology
- Published
- 2024-09-17
- DOI
- https://doi.org/10.3389/fmicb.2024.1442797
- Citations
- 2
- Primary Topic
- Biofuel production and bioconversion
- Type
- article
- Field-Weighted Citation Impact
- 0.37
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Gansu Province
- Gansu Agricultural University
- Science and Technology Department of Gansu Province
- Gansu Academy of Sciences