Aqueous extraction of formalin-fixed paraffin-embedded tissue and detection of prion disease using real-time quaking-induced conversion
We found complete agreement between the TSE status and the results from RT-QuIC seeded with the aqueous extract of FFPET samples. The method affords the diagnostic assessment TSE status by RT-QuIC of FFPET without the use of organic solvents that would otherwise create a mixed chemical-biological waste for disposal.
Authors
- Justin J. Greenlee (ORCID: https://orcid.org/0000-0003-2202-3054)
- Soyoun Hwang
- Eric M. Nicholson (ORCID: https://orcid.org/0000-0002-2217-0161)
Institutions
- Center for Biologics Evaluation and Research (US)
- National Animal Disease Center (US)
Publication Details
- Journal
- BMC Research Notes
- Published
- 2024-09-16
- DOI
- https://doi.org/10.1186/s13104-024-06886-6
- Citations
- 2
- Primary Topic
- Prion Diseases and Protein Misfolding
- Type
- article
- Field-Weighted Citation Impact
- 0.47
Funders
- U.S. Department of Agriculture
- Agricultural Research Service