Dynamics and necessity of SIRT1 for maternal–zygotic transition
Dynamic changes in maternal‒zygotic transition (MZT) require complex regulation of zygote formation, maternal transcript decay, embryonic genome activation (EGA), and cell cycle progression. Although these changes are well described, some key regulatory factors are still elusive. Sirtuin-1 (SIRT1), an NAD<sup>+</sup>-dependent histone deacetylase, is a versatile driver of MZT via its epigenetic and nonepigenetic substrates. This study focused on the dynamics of SIRT1 in early embryos and its contribution to MZT. A conditional SIRT1-deficient knockout mouse model was used, accompanied by porcine and human embryos. Embryos across mammalian species showed the prominent localization of SIRT1 in the nucleus throughout early embryonic development. Accordingly, SIRT1 interacts with histone H4 on lysine K16 (H4K16) in both mouse and human blastocysts. While maternal SIRT1 is dispensable for MZT, at least one allele of embryonic Sirt1 is required for early embryonic development around the time of EGA. This role of SIRT1 is surprisingly mediated via a transcription-independent mode of action.
Authors
- Milena Králíčková (ORCID: https://orcid.org/0000-0003-1907-4774)
- Jaroslav Petr (ORCID: https://orcid.org/0000-0002-7775-149X)
- Jiřina Havránková
- Jan Kubovčiak (ORCID: https://orcid.org/0000-0001-6446-9797)
- Michal Benc (ORCID: https://orcid.org/0000-0002-1241-8266)
- František Liška (ORCID: https://orcid.org/0000-0002-9588-806X)
- Miki Shavit
- Petr Hošek (ORCID: https://orcid.org/0000-0002-9359-4770)
- Michaela Vaškovičová (ORCID: https://orcid.org/0000-0003-4928-5732)
- Dávid Drutovič (ORCID: https://orcid.org/0000-0001-7099-3042)
- María Iniesta‐Cuerda (ORCID: https://orcid.org/0000-0002-2802-1925)
- František Strejček (ORCID: https://orcid.org/0000-0003-2268-3263)
- Tereza Žalmanová
- Jan Nevoral (ORCID: https://orcid.org/0000-0002-2048-7325)
- Ladan Monsef
- Iveta Valentova
- Sara Stachovicova
Institutions
- Ministry of Industry and Trade (CZ)
- Université de Versailles Saint-Quentin-en-Yvelines (FR)
- Czech Academy of Sciences (CZ)
- Charles University (CZ)
- Université Paris-Saclay (FR)
- Constantine the Philosopher University in Nitra (SK)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Institute of Animal Science (CZ)
- Czech Academy of Sciences, Institute of Animal Physiology and Genetics (CZ)
- Institute of Molecular Genetics (RU)
- Czech Academy of Sciences, Institute of Molecular Genetics (CZ)
Publication Details
- Journal
- Scientific Reports
- Published
- 2024-09-16
- DOI
- https://doi.org/10.1038/s41598-024-72595-6
- Citations
- 5
- Primary Topic
- Sirtuins and Resveratrol in Medicine
- Type
- article
- Field-Weighted Citation Impact
- 1.72
Funders
- Univerzita Karlova v Praze
- Ministerstvo Školství, Mládeže a Tělovýchovy
- Grantová Agentura České Republiky
- Technologická Agentura České Republiky
- Ministerstvo Zdravotnictví Ceské Republiky
- Agentúra na Podporu Výskumu a Vývoja
- Vedecká Grantová Agentúra MŠVVaŠ SR a SAV
- Ministerstvo Zemědělství
- Všeobecná Fakultní Nemocnice v Praze