Assessment of eruca sativa leaves extract ZnO NPs effect on the adverse effects of creatine - induced testes injury

This study was conducted to evaluate the protective role of Eruca sativa NPs on the harmful effects of creatine on the testes in male rats. This study used 48 male rats, in two experiments. The first experiment included determining the most effective nano-concentration of watercress extract, while the second experiment included evaluating the role of the nano-extract at the concentration that was chosen based on the results of the first experiment (60 mg/kg) against toxicity resulting from doses of creatine (4 g/kg) for 60 days. This study used 24 adult male rats were randomly assigned to four groups, with six animals per group, and were treated as follows:(G1) 1 ml of 0.09% physiological solution , (G2) dosed (single dose/day) with nano-extract of Eruca sativa at a concentration of (60 mg/kg) , (G3) The animals were dosed (single dose/day) with creatine monohydrate (4 g/kg), (G4 prevention group) dosed with nano-extract of Eruca sativa (60 mg/kg) followed one hour later by a dose of creatine (4 g/kg) . The results of the study indicated a non significant in Concentration of sperm in the epididymis, Percentage of live sperm ,Percentage of motile sperm , Percentage of normal sperm, LH , FSH and Testosterone in G2 and G4 compared to G1. As for the preventive group G4, it recorded a normal results near from control group. it recorded a significant decrease (P<0.05) in Concentration of sperm in the epididymis, Percentage of live sperm ,Percentage of motile sperm , Percentage of normal sperm, LH , FSH and Testosterone in G3 compared to G1 and G2 . The results of the histological examination in G3 showed that there are interstitial spaces between the seminiferous tubules and few sperm in the cavities of the tubules with reduction and disintegration of the interstitial tissue and a lack of Leydig cells and a decrease in the size of the germinal epithelial cell layer with cell degeneration necrosis of cells lining the tubules compared to G1,G2 and G4. The results of this article revealed the positive effects of Eruca sativa leaf ZnO NPs extract on the adverse effects of creatine-induced testes injury.