Near-infrared <i>in vivo</i> imaging system for dynamic visualization of lung-colonizing bacteria in mouse pneumonia
Conventional animal models of infectious diseases have traditionally relied upon average assessments involving numerous individuals, meaning they do not directly reflect changes in the pathology of an individual. Moreover, in recent years, ethical concerns have resulted in the demand to reduce the number of animals used in such models. Although <i>in vivo</i> imaging offers an effective approach for longitudinally evaluating the pathogenesis of infectious diseases in individual animals, a standardized method has not yet been established. To our knowledge, this study is the first to develop a highly versatile <i>in vivo</i> pulmonary bacterial quantification system utilizing near-infrared luminescence, plasmid-mediated expression of firefly luciferase in bacteria, and a scientific complementary metal-oxide semiconductor camera. Our research holds promise as a useful tool for assessing the efficacy of therapeutic drugs and pathogenesis of infectious diseases.
Authors
- Yuji Morita (ORCID: https://orcid.org/0000-0003-1371-8591)
- Noriteru Yamada
- Ryuichi Nakano (ORCID: https://orcid.org/0000-0003-2086-2591)
- Hisakazu Yano (ORCID: https://orcid.org/0000-0003-2085-7194)
- Daiki Yamaguchi
- Shôjirô Maki (ORCID: https://orcid.org/0000-0002-9741-0166)
- Nobuo Kitada (ORCID: https://orcid.org/0000-0002-3104-6963)
- Kinnosuke Yahiro (ORCID: https://orcid.org/0000-0002-9813-0780)
- Go Kamoshida (ORCID: https://orcid.org/0000-0001-6300-0911)
- Ryohei Saito‐Moriya
- Shinichi Kato (ORCID: https://orcid.org/0000-0002-8280-7558)
- Syun Kawakubo
- Ayane Okuda
- Keisuke Ueyama
- R. Tanaka
- Saki Azuma
- Takamitsu Tsuji
- S Isaka
- Manaha Tomita
Institutions
- University of Electro-Communications (JP)
- Meiji Pharmaceutical University (JP)
- Kyoto Pharmaceutical University (JP)
- Japan Women's University (JP)
- Nara Medical University (JP)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2024-09-17
- DOI
- https://doi.org/10.1128/spectrum.00828-24
- Citations
- 3
- Primary Topic
- bioluminescence and chemiluminescence research
- Type
- article
- Field-Weighted Citation Impact
- 0.70
Funders
- Japan Agency for Medical Research and Development
- National Center for Global Health and Medicine
- Kyoto Pharmaceutical University
- Ichiro Kanehara Foundation for the Promotion of Medical Sciences and Medical Care
- Japan Society for the Promotion of Science