3D Printing as a Strategy to Scale-Up Biohybrid Hydrogels for T Cell Manufacture
The emergence of cellular immunotherapy treatments is introducing more efficient strategies to combat cancer as well as autoimmune and infectious diseases. However, the cellular manufacturing procedures associated with these therapies remain costly and time-consuming, thus limiting their applicability. Recently, lymph-node-inspired PEG-heparin hydrogels have been demonstrated to improve primary human T cell culture at the laboratory scale. To go one step further in their clinical applicability, we assessed their scalability, which was successfully achieved by 3D printing. Thus, we were able to improve primary human T cell infiltration in the biohybrid PEG-heparin hydrogels, as well as increase nutrient, waste, and gas transport, resulting in higher primary human T cell proliferation rates while maintaining the phenotype. Thus, we moved one step further toward meeting the requirements needed to improve the manufacture of the cellular products used in cellular immunotherapies.
Authors
- Miguel A. Mateos‐Timoneda (ORCID: https://orcid.org/0000-0001-7657-1414)
- Judith Guasch (ORCID: https://orcid.org/0000-0002-3571-4711)
- Fabião Santos (ORCID: https://orcid.org/0000-0003-4261-9672)
- Elisabeth Engel (ORCID: https://orcid.org/0000-0003-4855-8874)
- Sergi Rey-Viñolas (ORCID: https://orcid.org/0000-0002-2128-7704)
- Imma Ratera (ORCID: https://orcid.org/0000-0002-1464-9789)
- Jaume Veciana (ORCID: https://orcid.org/0000-0003-1023-9923)
- Eduardo Pérez del Río (ORCID: https://orcid.org/0000-0003-2444-5621)
- Miquel Castellote‐Borrell (ORCID: https://orcid.org/0000-0001-7703-2228)
- Francesca Merlina (ORCID: https://orcid.org/0000-0001-5040-1687)
Institutions
- Universitat Internacional de Catalunya (ES)
- Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (ES)
- Institut de Ciència de Materials de Barcelona (ES)
- Institute for Bioengineering of Catalonia (ES)
- Universitat Politècnica de Catalunya (ES)
Publication Details
- Journal
- ACS Applied Materials & Interfaces
- Published
- 2024-09-17
- DOI
- https://doi.org/10.1021/acsami.4c06183
- Citations
- 6
- Primary Topic
- CAR-T cell therapy research
- Type
- article
- Field-Weighted Citation Impact
- 1.70
Funders
- Ministerio de Ciencia, Innovación y Universidades
- European Commission
- Generalitat de Catalunya
- Ministerio de Economía y Competitividad
- Max-Planck-Gesellschaft
- Ministerio de Ciencia e Innovación
- Horizon 2020 Framework Programme
- Instituto de Salud Carlos III
- European Regional Development Fund
- H2020 Marie Skłodowska-Curie Actions