Volatile organic compound profile for the search of rejection markers in protein baits used as Vespa velutina control method

• VOCs profiles of protein baits for Vespa velutina control was obtained by SPME-GC-MS. • PCA allowed differentiating the protein baits according to the different storage conditions. • The field assays provided the optimal storage conditions in which the protein baits are attracted/rejected by hornets. • The rejection markers belong to the families of ketones, esters, alcohols and amines were obtained by chemometric tools. The invasive species Vespa velutina nigrithorax ( Lepeletier 1836), commonly known as the Asian hornet, constitutes an economic and environmental threat, as it is a predator of fruits and insects, especially Apis mellifera ( Linnaeus , 1758) (honey bees). Different methods have been developed for its control. Among of them, biocidal protein baits are included, which have a limited time of use. Oxidation of their components, enzymatic activity and microorganisms lead to the formation of spoilage compounds. The aim of this work was to determine the optimal conditions for storing and preserving the baits without losing efficacy. Furthermore, a search of rejection markers will be carried out based on field studies results in the apiary on the acceptance and rejection of the baits by hornets under different storage conditions. Volatile organic compound profiles of these baits were obtained by solid phase microextraction/gas chromatography/mass spectrometry (SPME-GC–MS). The data were processed using different statistical and chemometric tools. The identification of potential rejection markers belonging to the families of ketones, esters, alcohols and amines was carried out by partial least squares discriminant analysis (PLS-DA). The four markers with the highest VIP scores were 2-butanone, 2-heptanone, 1-butanol, 3-methyl and 1-butanol, 3-methyl, acetate.