An optimised method to isotopically label pure synthetic peptides ‘in‐house’ for absolute quantification in bottom‐up proteomics
The application of the 'in-house' labelled standards in generating calibration curves to quantify endogenous peptide concentrations is just as effective as using the synthetically sourced standards while also having great cost reduction implications as well as saving time spent waiting for peptides to arrive. The protocol is highly adaptable and can be customized to fit the specific setup of any laboratory, maximizing achievable labelling efficiencies.
Authors
- Nikita Bhakta (ORCID: https://orcid.org/0009-0003-9238-6675)
- Emer M. Brady (ORCID: https://orcid.org/0000-0002-4715-9145)
- Donald J. L. Jones (ORCID: https://orcid.org/0000-0001-6583-870X)
- Jatinderpal K. Sandhu (ORCID: https://orcid.org/0000-0002-5612-274X)
- Colleen B. Maxwell (ORCID: https://orcid.org/0000-0003-4463-3566)
- Oliver C. Slingsby (ORCID: https://orcid.org/0000-0002-3355-5163)
- Leong L. Ng (ORCID: https://orcid.org/0000-0002-6553-5749)
- Shimon Atunde
Institutions
- University of Leicester (GB)
- Glenfield Hospital (GB)
- NIHR Leicester Biomedical Research Centre (GB)
Publication Details
- Journal
- Rapid Communications in Mass Spectrometry
- Published
- 2024-09-17
- DOI
- https://doi.org/10.1002/rcm.9892
- Primary Topic
- Advanced Proteomics Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institute for Health and Care Research
- Department of Health and Social Care
- University of Leicester
- John and Lucille Van Geest Foundation
- Medical Research Council
- NIHR Leicester Biomedical Research Centre