Synthesis of novel selenium nanohybrid for biotechnology and risk assessment of multi-drug resistance bacteria

Relatively recently, the significance of nanomaterials in several domains like materials science, chemistry, and biology has increased dramatically. Selenium is a vital trace element of enormous importance involved in electronics, sensors, catalysis, optics, and biological applications. The study's objective was to synthesize an enterocin conjugate with selenium nanoparticles to form a nanohybrid system that may increase the bioactivity of enterocin and overcome these hurdles. The nanohybrid system was characterized using the use of UV–vis, FTIR, XRD, EDX, TEM, Zeta potential and SEM. This system was used to health one risk assessment multi-drug resistance bacteria. The designed nanohybrid system showed high ability to growth inhibition of all these bacteria. As a result, the nanohybrid showed strong antibacterial activity against MDR bacteria using several accepted techniques. The biocidal effects of nanohybrid on tested bacteria was investigated using SEM. Upon examination, the results revealed damage, blebs, fusion, clumping, and uneven distribution in the cell wall of the tested bacteria, ultimately leading to cell death. The results showed that the system is good engineering design to kill bacteria. In addition, compared with bacteriocin alone, the nanohybrid showed promising antioxidant activity in vitro. • Conjugation between Enterococcus faecium Enterocinand selenium nanoparticles to form a novel nanohybrid system. • The designed nanohybrid system showed high ability to growth inhibition of MDR bacteria. • The results showed that the system is good engineering design to kill bacteria. • Nanohybrid system exhibited promising biotechnology activities.