Bornite (Cu5FeS4) nanocrystals as an ultrasmall biocompatible NIR-II contrast agent for photoacoustic imaging
In this study, we demonstrate the potential of the bornite crystal structure (Cu<sub>5</sub>FeS<sub>4</sub>) of copper iron sulfide as a second near infrared (NIR-II) photoacoustic (PA) contrast agent. Bornite exhibits comparable dose-dependent biocompatibility to copper sulfide nanoparticles in a cell viability study with HepG2 cells, while exhibiting a 10-fold increase in PA amplitude. In comparison to other benchmark contrast agents at similar mass concentrations, bornite demonstrated a 10× increase in PA amplitude compared to indocyanine green (ICG) and a 5× increase compared to gold nanorods (AuNRs). PA signal was detectable with a light pathlength greater than 5 cm in porcine tissue phantoms at bornite concentrations where in vitro cell viability was maintained. In vivo imaging of mice vasculature resulted in a 2× increase in PA amplitude compared to AuNRs. In summary, bornite is a promising NIR-II contrast agent for deep tissue PA imaging.
Authors
- Kristie Huda
- Xingjian Zhong (ORCID: https://orcid.org/0000-0002-9133-0018)
- Allison M. Dennis (ORCID: https://orcid.org/0000-0001-5759-9905)
- Joshua Kays (ORCID: https://orcid.org/0000-0002-9682-6792)
- Carolyn L. Bayer (ORCID: https://orcid.org/0000-0003-0947-6892)
- Swathi P. Katakam
- Vinoin Devpaul Vincely (ORCID: https://orcid.org/0000-0003-4844-5984)
Institutions
- Boston University (US)
- Tulane University (US)
- Northeastern University (US)
Publication Details
- Journal
- Photoacoustics
- Published
- 2024-09-17
- DOI
- https://doi.org/10.1016/j.pacs.2024.100649
- Citations
- 6
- Primary Topic
- Photoacoustic and Ultrasonic Imaging
- Type
- article
- Field-Weighted Citation Impact
- 1.11
Funders
- Chan Zuckerberg Initiative
- National Institutes of Health
- National Institute of General Medical Sciences
- National Institute of Child Health and Human Development