Protocol to process fresh human cerebral cortex biopsies for patch-clamp recording and immunostaining
Cerebral cortex biopsies enable the investigation of native developing and mature human brain tissue. Here, we present a protocol to process human cortical biopsies from the surgical theater to the laboratory. We describe steps for the preparation of viable acute slices for patch-clamp recording using dedicated chemical solutions for transport and sectioning. We then explain procedures for tissue fixation and post hoc immunostaining to correlate physiological properties to morphological features and protein detection. For complete details on the use and execution of this protocol, please refer to Libé-Philippot et al.<sup>1</sup>.
Authors
- Hajnalka Nyitrai
- Ine Vlaeminck (ORCID: https://orcid.org/0000-0001-8934-6179)
- Amélie Lejeune
- Tom Theys (ORCID: https://orcid.org/0000-0001-7595-3234)
- Baptiste Libé‐Philippot (ORCID: https://orcid.org/0000-0001-8283-804X)
- Joris de Wit (ORCID: https://orcid.org/0000-0003-1120-1089)
- Pierre Vanderhaeghen (ORCID: https://orcid.org/0000-0001-7899-5720)
- Keimpe Wierda (ORCID: https://orcid.org/0000-0002-8784-9490)
- Elke Leysen (ORCID: https://orcid.org/0000-0002-5606-6725)
Institutions
- Allen Institute for Brain Science (US)
- VIB-KU Leuven Center for Brain & Disease Research (BE)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-09-17
- DOI
- https://doi.org/10.1016/j.xpro.2024.103313
- Citations
- 4
- Primary Topic
- Cell Image Analysis Techniques
- Type
- article
- Field-Weighted Citation Impact
- 2.65
Funders
- Universiteit van Amsterdam
- Fonds De La Recherche Scientifique - FNRS
- Fonds Wetenschappelijk Onderzoek
- KU Leuven
- Onderzoeksraad, KU Leuven
- Vlaamse regering
- Universitaire Ziekenhuizen Leuven, KU Leuven