Multi-omics analysis using antibody-based in situ biotinylation technique suggests the mechanism of Cajal body formation
Membrane-less subcellular compartments play important roles in various cellular functions. Although techniques exist to identify components of cellular bodies, a comprehensive method for analyzing both static and dynamic states has not been established. Here, we apply an antibody-based in situ biotinylation proximity-labeling technique to identify components of static and dynamic nuclear bodies. Using this approach, we comprehensively identify DNA, RNA, and protein components of Cajal bodies (CBs) and then clarify their interactome. By inhibiting transcription, we capture dynamic changes in CBs. Our analysis reveals that nascent small nuclear RNAs (snRNAs) transcribed in CBs contribute to CB formation by assembling RNA-binding proteins, including frontotemporal dementia-related proteins, RNA-binding motif proteins, and heterogeneous nuclear ribonucleoproteins.
Authors
- Yuki Yamaguchi (ORCID: https://orcid.org/0000-0002-0197-614X)
- Nobuyoshi Akimitsu (ORCID: https://orcid.org/0000-0002-3190-2942)
- Akihide Ryo (ORCID: https://orcid.org/0000-0001-6028-8091)
- Atsushi Toyoda (ORCID: https://orcid.org/0000-0002-0728-7548)
- Hidehisa Takahashi (ORCID: https://orcid.org/0000-0001-7181-9863)
- Yoko Ike
- Ryota Abe
- Yayoi Kimura (ORCID: https://orcid.org/0000-0002-4612-4602)
- Rena Onoguchi‐Mizutani
- Yoko Ino
- Keiko Horiuchi (ORCID: https://orcid.org/0000-0003-4811-5133)
- Keisuke Noguchi (ORCID: https://orcid.org/0000-0002-3130-7828)
- Hiroshi Doi (ORCID: https://orcid.org/0000-0003-3237-2119)
- Fumiaki Tanaka (ORCID: https://orcid.org/0000-0002-0968-8871)
- Hidefumi Suzuki
- Tomohiko Akiyama (ORCID: https://orcid.org/0000-0002-1238-8095)
- Yutaka Suzuki
- Shintaro Ogawa (ORCID: https://orcid.org/0000-0002-5203-9324)
Institutions
- National Institute of Genetics (JP)
- Tokyo Institute of Technology (JP)
- Yokohama City University Medical Center (JP)
- National Institute of Infectious Diseases (JP)
- The University of Tokyo (JP)
- Yokohama City University (JP)
Publication Details
- Journal
- Cell Reports
- Published
- 2024-09-01
- DOI
- https://doi.org/10.1016/j.celrep.2024.114734
- Citations
- 6
- Primary Topic
- Biotin and Related Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.96
Funders
- Japan Agency for Medical Research and Development
- Japan Society for the Promotion of Science