Serum D-asparagine concentration adjusted for eGFR could serve as a novel screening tool for urothelial carcinoma
The sensitivity of currently available screening tools for urothelial carcinoma (UC) remains unsatisfactory particularly at early stages. Hence, we aimed to establish a novel blood-based screening tool for urothelial carcinoma. We measured serum d-amino acid levels in 108 and 192 patients with and without UC individuals in the derivation cohort, and 15 and 25 patients with and without UC in the validation cohort. Serum d-asparagine levels were significantly higher in patients with UC than in those without UC (p < 0.0001). We developed a novel screening equation for the diagnosis of urothelial carcinoma using d-asparagine in serum and estimated the glomerular filtration rate (eGFR). Serum d-asparagine levels adjusted for eGFR exhibited high performance in the diagnosis of UC (AUC-ROC, 0.869; sensitivity, 80.6 %; specificity, 82.7 %), even in early-stage UC (AUC-ROC: 0.859, sensitivity: 83.3 %, specificity: 82.3 %), which were previously misdiagnosed via urinary occult blood or urine cytology. This established strategy combined with urinary occult blood, improves diagnostic ability (sensitivity: 93.7 %, specificity: 70.1 %).
Authors
- Yoichi Kakuta (ORCID: https://orcid.org/0000-0001-5339-4312)
- Akinaru Yamamoto (ORCID: https://orcid.org/0000-0002-3565-5870)
- Akihiro Yoshimura (ORCID: https://orcid.org/0000-0001-9059-2318)
- Masaru Tani (ORCID: https://orcid.org/0009-0001-3079-0520)
- Toshihiro Uemura
- Koji Hatano (ORCID: https://orcid.org/0000-0002-8409-5152)
- Atsunari Kawashima (ORCID: https://orcid.org/0000-0001-9369-4264)
- Masashi Mita (ORCID: https://orcid.org/0000-0002-9379-6006)
- Yu Ishizuya (ORCID: https://orcid.org/0000-0003-0778-1308)
- Takuji Hayashi (ORCID: https://orcid.org/0000-0003-2030-3087)
- Gaku Yamamichi
- Yoshiyuki Yamamoto (ORCID: https://orcid.org/0000-0002-2932-5904)
- Ryoichi Imamura (ORCID: https://orcid.org/0000-0002-8782-9852)
- Taigo Kato (ORCID: https://orcid.org/0000-0002-8681-1407)
- Yuki Horibe (ORCID: https://orcid.org/0000-0002-4809-9498)
- Tomonori Kimura (ORCID: https://orcid.org/0000-0001-8186-094X)
- Norio Nonomura (ORCID: https://orcid.org/0000-0002-6522-6233)
- Shunsuke Inoguchi
- Yutong Liu (ORCID: https://orcid.org/0009-0009-3844-9456)
- Nesrine Sassi
- Shinsuke Sakai
- Shiro Takahara
- Yohei Okuda (ORCID: https://orcid.org/0000-0001-9909-4200)
- Toshiki Oka (ORCID: https://orcid.org/0000-0002-2023-7211)
Institutions
- Kansai Medical University (JP)
- Kagami Crystal (Japan) (JP)
- Nagasaki University (JP)
- The University of Osaka (JP)
Publication Details
- Journal
- Biochemical and Biophysical Research Communications
- Published
- 2024-09-14
- DOI
- https://doi.org/10.1016/j.bbrc.2024.150701
- Citations
- 1
- Primary Topic
- Bladder and Urothelial Cancer Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.57
Funders
- Takeda Science Foundation
- Takeda Medical Research Foundation
- Japan Society for the Promotion of Science