Quantitative separation of CEST effect by Rex-line-fit analysis of Z-spectra

The process of chemical exchange saturation transfer (CEST) is quantified by evaluating a Z-spectra, where CEST signal quantification and Z-spectra fitting have been widely used to distinguish the contributions from multiple origins. Based on the exchange-dependent relaxation rate in the rotating frame (R_ex), this paper introduces an additional pathway to quantitative separation of CEST effect. The proposed R_ex-line-fit method is solved by a multi-pool model and presents the advantage of only being dependent of the specific parameters (solute concentration, solute-water exchange rate, solute transverse relaxation, and irradiation power). Herein we show that both solute-water exchange rate and solute concentration monotonously vary with R_ex for Amide, Guanidino, NOE and MT, which has the potential to assist in solving quantitative separation of CEST effect. Furthermore, we achieve R_ex imaging of Amide, Guanidino, NOE and MT, which may provide direct insight into the dependency of measurable CEST effects on underlying parameters such as the exchange rate and solute concentration, as well as the solute transverse relaxation.