Development of biological techniques to prevent corrosion of reinforcing steel bars

The electrochemical corrosive processes compromise the passivity of reinforcing steel, potentially leading to structural integrity loss and, in extreme cases, concrete infrastructure failures. While bio-inspired concretes show promise in mitigating strength degradation and enabling self-healing of concrete flaws, their interaction with steel reinforcement remains underexplored. Thus, this investigation aimed to establish a protective strategy by fostering biofilm growth on rebar surfaces. To achieve this, <i>Bacillus subtilis</i> and <i>Escherichia coli</i> bacteria were utilized as biofilm-forming agents, aided by magnetic iron oxide and zeolite micro-nano particles. The study encompassed a thorough assessment of split tensile strength, corrosion resistance of bio-treated embedded steel bars, and a comprehensive biofilm characterization, along with a meticulous examination of the microstructure at the steel-concrete interface. The findings underscored a significant improvement in split tensile strength, demonstrating a remarkable 84.2 % increase when bacterial species were combined with iron oxide nanoparticles, in contrast to the control specimens. Furthermore, the bio-treated bars exhibited an impressive corrosion inhibition potential of 78.5 % relative to their unaltered counterparts. These outcomes are attributed to the discernible refinement of microstructural features surrounding the steel reinforcement and the heightened densification of the inter-transitional zone between steel and concrete.