Areal specializations in the morpho-electric and transcriptomic properties of primate layer 5 extratelencephalic projection neurons
Large-scale analysis of single-cell gene expression has revealed transcriptomically defined cell subclasses present throughout the primate neocortex with gene expression profiles that differ depending upon neocortical region. Here, we test whether the interareal differences in gene expression translate to regional specializations in the physiology and morphology of infragranular glutamatergic neurons by performing Patch-seq experiments in brain slices from the temporal cortex (TCx) and motor cortex (MCx) of the macaque. We confirm that transcriptomically defined extratelencephalically projecting neurons of layer 5 (L5 ET neurons) include retrogradely labeled corticospinal neurons in the MCx and find multiple physiological properties and ion channel genes that distinguish L5 ET from non-ET neurons in both areas. Additionally, while infragranular ET and non-ET neurons retain distinct neuronal properties across multiple regions, there are regional morpho-electric and gene expression specializations in the L5 ET subclass, providing mechanistic insights into the specialized functional architecture of the primate neocortex.
Authors
- Steve I. Perlmutter (ORCID: https://orcid.org/0000-0002-1149-4152)
- Naz Taskin (ORCID: https://orcid.org/0009-0003-2417-8428)
- Brian Kalmbach (ORCID: https://orcid.org/0000-0003-3136-8097)
- Natalie Weed (ORCID: https://orcid.org/0000-0003-0891-0327)
- Jonathan T. Ting (ORCID: https://orcid.org/0000-0001-8266-0392)
- Ximena Opitz-Araya (ORCID: https://orcid.org/0000-0003-4960-3622)
- Nelson Johansen (ORCID: https://orcid.org/0000-0002-4436-969X)
- Jeremy A. Miller (ORCID: https://orcid.org/0000-0003-4549-588X)
- Jeff Goldy (ORCID: https://orcid.org/0000-0001-5140-6922)
- K. K. Nasirova
- Nikolai Dembrow (ORCID: https://orcid.org/0000-0002-7699-6591)
- Michael Tieu (ORCID: https://orcid.org/0000-0001-9286-5623)
- Staci A. Sorensen (ORCID: https://orcid.org/0000-0002-6799-2126)
- Lauren Alfiler (ORCID: https://orcid.org/0000-0003-4847-7900)
- Kimberly A. Smith (ORCID: https://orcid.org/0000-0002-3142-1970)
- Julia Wilson (ORCID: https://orcid.org/0000-0001-6155-5374)
- Sarah Walling-Bell (ORCID: https://orcid.org/0000-0001-6995-2681)
- Scott F. Owen (ORCID: https://orcid.org/0000-0001-6294-7513)
- Cristina Radaelli (ORCID: https://orcid.org/0000-0002-3255-2630)
- William J. Spain (ORCID: https://orcid.org/0000-0001-9213-7335)
- Ed S. Lein (ORCID: https://orcid.org/0000-0001-9012-6552)
- Rachel Dalley (ORCID: https://orcid.org/0000-0001-7461-7845)
- Darren Bertagnolli (ORCID: https://orcid.org/0000-0002-6626-1567)
- David Vumbaco (ORCID: https://orcid.org/0000-0001-8115-3081)
- Brian Lee (ORCID: https://orcid.org/0000-0002-3210-5638)
- Alejandro Parga-Becerra
- Scott Sawchuk
- Mark Hudson
Institutions
- Center for Infectious Disease Research (US)
- Allen Institute for Brain Science (US)
- University of Washington (US)
- University of Washington Medical Center (US)
- Seattle Children's Hospital (US)
- Allen Institute (US)
Publication Details
- Journal
- Cell Reports
- Published
- 2024-09-01
- DOI
- https://doi.org/10.1016/j.celrep.2024.114718
- Citations
- 9
- Primary Topic
- Neural dynamics and brain function
- Type
- article
- Field-Weighted Citation Impact
- 3.08
Funders
- National Institutes of Health
- National Center for Advancing Translational Sciences
- Office of Research Infrastructure Programs, National Institutes of Health