Molecular and in silico analyses for detection of Shiga toxin-producing Escherichia coli (STEC) and highly pathogenic enterohemorrhagic Escherichia coli (EHEC) using genetic markers located on plasmid, O Island 57 and O Island 71
Overall, using a single gene marker, Z2098, Z2099, and ecf1 are sensitive targets for screening the top 7 STEC isolates, and the combination of Z2098/Z2099 offers a more targeted initial screening method to detect the top 7 STEC isolates. Detecting non-top 7 STEC in both animal and human samples proved challenging due to inconsistent characteristics associated with the genetic markers studied.
Authors
- Stefano Morabito (ORCID: https://orcid.org/0000-0003-2467-2912)
- Ali Dadvar (ORCID: https://orcid.org/0009-0001-0938-5125)
- Mahdi Askari Badouei (ORCID: https://orcid.org/0000-0001-5051-813X)
- Gholamreza Hashemi Tabar (ORCID: https://orcid.org/0000-0001-9760-1096)
- Ali Nemati (ORCID: https://orcid.org/0000-0002-8093-4713)
Institutions
- European Union (BE)
- Ferdowsi University of Mashhad (IR)
Publication Details
- Journal
- BMC Veterinary Research
- Published
- 2024-09-13
- DOI
- https://doi.org/10.1186/s12917-024-04251-0
- Citations
- 7
- Primary Topic
- Escherichia coli research studies
- Type
- article
- Field-Weighted Citation Impact
- 4.32
Funders
- Ferdowsi University of Mashhad