Comparison of multi-parallel quantitative real-time PCRs targeting different DNA regions and detecting soil-transmitted helminths in stool
There is a strong correlation between qPCR results and egg/larvae counts. Our study confirms that qPCR is an effective diagnostic tool, even with low-intensity infections, regardless of the DNA-based diagnostic marker used. However, the moderate agreement between the two different qPCR assays when testing field samples highlights the need to understand the role of these targets in the genome so that the parasite burden can be quantified more accurately and consistently by qPCR.
Authors
- D. Timothy J. Littlewood (ORCID: https://orcid.org/0000-0002-2718-4001)
- Rojelio Mejía (ORCID: https://orcid.org/0000-0003-4134-4558)
- Julia C. Dunn (ORCID: https://orcid.org/0000-0002-4789-9258)
- Nils Pilotte (ORCID: https://orcid.org/0000-0002-8447-7425)
- Alejandro Krolewiecki (ORCID: https://orcid.org/0000-0001-9054-1211)
- Steven A. Williams (ORCID: https://orcid.org/0000-0002-4881-7496)
- Marina Papaiakovou (ORCID: https://orcid.org/0000-0001-9012-2703)
- Rubén O. Cimino (ORCID: https://orcid.org/0000-0002-4253-9077)
Institutions
- National University of Salta (AR)
- Natural History Museum (GB)
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Smith College (US)
- University of Cambridge (GB)
- Texas Children's Hospital (US)
- Quinnipiac University (US)
- Imperial College London (GB)
Publication Details
- Journal
- Parasites & Vectors
- Published
- 2024-09-13
- DOI
- https://doi.org/10.1186/s13071-024-06464-6
- Citations
- 1
- Primary Topic
- Parasitic Infections and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 0.52
Funders
- United States Agency for International Development
- Bill and Melinda Gates Foundation
- Government of the United Kingdom