A multi-center study on genetic variations in the fusion protein of respiratory syncytial virus from children with Acute Lower Respiratory Tract Infections in China during 2017–2021
Respiratory syncytial virus (RSV) is a significant cause of acute lower respiratory tract infection (ALRTI) in children under five years of age. Between 2017 and 2021, 396 complete sequences of the RSV F gene were obtained from 500 RSV-positive throat swabs collected from ten hospitals across nine provinces in China. In addition, 151 sequences from China were sourced from GenBank and GISAID, making a total of 549 RSV F gene sequences subjected to analysis. Phylogenetic and genetic diversity analyses revealed that the RSV F genes circulating in China from 2017 to 2021 have remained relatively conserved, although some amino acids (AAs) have undergone changes. AA mutations with frequencies ≥ 10% were identified at six sites and the p27 region: V384I (site I), N276S (site II), R213S (site Ø), and K124N (p27) for RSV A; F45L (site I), M152I/L172Q/S173 L/I185V/K191R (site V), and R202Q/I206M/Q209R (site Ø) for RSV B. Comparing mutational frequencies in RSV-F before and after 2020 revealed minor changes for RSV A, while the K191R, I206M, and Q209R frequencies increased by over 10% in RSV B. Notably, the nirsevimab-resistant mutation, S211N in RSV B, increased in frequency from 0% to 1.15%. Both representative strains aligned with the predicted RSV-F structures of their respective prototypes exhibited similar conformations, with low root-mean-square deviation values. These results could provide foundational data from China for the development of RSV mAbs and vaccines.
Authors
- Xiangpeng Chen (ORCID: https://orcid.org/0000-0001-6955-6515)
- Lili Zhong (ORCID: https://orcid.org/0009-0009-1461-4517)
- Jikui Deng (ORCID: https://orcid.org/0000-0002-0040-1491)
- Yun Zhu (ORCID: https://orcid.org/0000-0003-3531-3544)
- Lili Xu (ORCID: https://orcid.org/0000-0002-6146-5130)
- Zhengde Xie (ORCID: https://orcid.org/0000-0001-7634-9338)
- Lei Li (ORCID: https://orcid.org/0000-0002-8828-5199)
- Gen Lu (ORCID: https://orcid.org/0000-0001-9702-6167)
- Fei Li (ORCID: https://orcid.org/0000-0002-0532-9054)
- Luci Huang (ORCID: https://orcid.org/0009-0002-1024-1097)
- Yiliang Fu
- Zhengxiu Luo (ORCID: https://orcid.org/0000-0003-2569-753X)
- Rong Jin (ORCID: https://orcid.org/0000-0002-5666-9497)
- Hailin Zhang (ORCID: https://orcid.org/0000-0002-7663-0418)
- Lingfeng Cao (ORCID: https://orcid.org/0000-0003-0450-7928)
- Ying Wu (ORCID: https://orcid.org/0000-0003-4035-7015)
- Hanwen Zhang (ORCID: https://orcid.org/0000-0003-3461-2972)
- Qiuping Li (ORCID: https://orcid.org/0000-0001-9363-7577)
Institutions
- Hunan Normal University (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Wenzhou Medical University (CN)
- Yinchuan First People's Hospital (CN)
- Shanghai Children's Medical Center (CN)
- Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University (CN)
- Guangzhou Women and Children Medical Center (CN)
- Beijing Children’s Hospital (CN)
- Shenzhen Children's Hospital (CN)
- Children's Hospital of Fudan University (CN)
- Children's Hospital of Chongqing Medical University (CN)
- Chongqing Medical University (CN)
- Guangzhou Medical University (CN)
Publication Details
- Journal
- Virologica Sinica
- Published
- 2024-09-13
- DOI
- https://doi.org/10.1016/j.virs.2024.09.002
- Citations
- 8
- Primary Topic
- Respiratory viral infections research
- Type
- article
- Field-Weighted Citation Impact
- 3.09
Funders
- Chinese Academy of Meteorological Sciences
- Beijing Municipal Health Commission
- National Key Research and Development Program of China
- National Major Science and Technology Projects of China