A Rhein-Based Derivative Targets Staphylococcus aureus

The rise in antibiotic-resistant bacteria highlights the need for novel antimicrobial agents. This study presents the design and synthesis of a series of rhein (RH)-derived compounds with improved antimicrobial properties. The lead compound, RH<b>17</b>, exhibited a potent antibacterial activity against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) isolates, with minimum inhibitory concentrations (MICs) ranging from 8 to 16 μg/mL. RH<b>17</b> disrupted bacterial membrane stability, hindered metabolic processes, and led to an increase in reactive oxygen species (ROS) production. These mechanisms were confirmed through bacterial growth inhibition assays, membrane function assessments, and ROS detection. Notably, RH<b>17</b> outperformed the parent compound RH and demonstrated bactericidal effects in <i>S. aureus</i>. The findings suggest that RH<b>17</b> is a promising candidate for further development as an antimicrobial agent against Gram-positive pathogens, addressing the urgent need for new therapies.