Chemometric classification of Vietnamese green tea ( <i>Camellia sinensis</i> ) varieties and origins using elemental profiling and FTIR spectroscopy

Abstract The objective of this study was to employ multi-elemental analysis and chemometric multivariate data analysis for identifying the geographical origin of Camellia sinensis cultivated in Vietnam's northeastern region. Ninety-two tea leaf samples encompassing four distinct varieties were sourced from Bac Can and Thai Nguyen provinces. These samples underwent analysis through Fourier transform infrared (FTIR) spectroscopy and chemometrics, while elemental quantification was achieved via inductively coupled plasma mass spectrometry (ICP-MS), utilising mixed standards for 26 elements. The FTIR spectra exhibited characteristic peaks, denoting the functional groups of the potential compounds present. ICP/MS analysis identified the presence of 14 elements in the tea samples, revealing significant variations in content across the four varieties. principal component analysis (PCA) of the FTIR data illustrated that the initial two principal components accounted for 91.26% of the total variance, effectively segregating the four green tea varieties into distinct clusters. Conversely, PCA of the ICP-MS spectra also distinguished the four varieties, albeit Trung Du and Hybrid F1 exhibited a proximate distribution. This closeness may be incidental and not indicative of correlation, considering the limited variable set. Our findings ascertain that ICP/MS and FTIR, augmented by chemometric software, serve as efficacious tools for ascertaining the geographical origins of tea.