Biocontrol of fungal pathogens and growth promotion in the Korean fir (Abies koreana E.H.Wilson) seedling using Bacillus velezensis CE 100

• Phomopsis and Fusarium species are devastating pathogens of Abies koreana seedling. • Bacillus velezensis demonstrated chitinolytic, proteolytic and glucanolytic potential. • B. velezensis degraded fungal cell, inhibited spore germination and mycelial growth. • The bacterium produced IAA and increased the availability of nitrogen and phosphate. • The bacterial treatment improved the survival and growth rate of A. koreana seedling. The restoration of the endangered Korean fir ( Abies koreana E.H.Wilson) forests requires mass production of high-quality and well-developed seedlings for outplanting. However, phytopathogenic fungal infections lower the survival rate and growth vigor of A. koreana seedlings, frustrating the re-afforestation efforts. This study isolated and identified the phytopathogenic fungi causing seedling blight and wilt diseases in A. koreana seedlings and investigated the potential of Bacillus velezensis CE 100 in controlling the fungal pathogens and promoting seedling growth. Phomopsis mali and Fusarium oxysporum were confirmed as the causal agents of blight and wilt diseases, respectively, and both fungi reduced the survival rate of A. koreana seedlings. B. velezensis CE 100 produced cell wall-degrading enzymes: chitinase, protease, and β-1,3-glucanase, and the bacterial crude enzyme fraction inhibited the spore germination and mycelial growth of P. mali and F. oxysporum in a concentration-dependent manner, causing severe morphological deformations. Consequently, treatment with B. velezensis CE 100 improved the survival rate of seedlings infected with either P. mali or F. oxysporum compared to the control and PB media treatment. The bacterium also increased nutrient availability through ammonia–nitrogen production and phosphate solubilization and produced indole-3-acetic acid (IAA), and its inoculation remarkably improved seedling growth (root and shoot dry weight) compared to the control and PB media treatment group. This is the first study to report P. mali and F. oxysporum as the pathogens causing seedling blight and wilt diseases in A. koreana nursery seedling, and the possibility of using B. velezensis CE 100 to control the fungal pathogens and improve the seedling growth without chemical use.