Development and validation of a multi-marker liquid bead array assay for the simultaneous detection of PIK3CA and ESR1 hotspot mutations in single circulating tumor cells (CTCs)
The developed novel multi-marker liquid bead array assay for the simultaneous detection of <i>PIK3CA</i> and <i>ESR1</i> hotspot mutations in single CTCs is highly specific, highly sensitive, high-throughput, and sample-, cost-, and time-saving. This multi-marker liquid bead array assay can be extended to detect up to 100 mutations in many genes at once and can be applied for bulk CTC and ctDNA analysis.
Authors
- Dimitra Stergiopoulou
- Evi Lianidou (ORCID: https://orcid.org/0000-0002-7796-5914)
- Athina Markou (ORCID: https://orcid.org/0000-0001-7760-9701)
- Vassilis Georgoulias
Institutions
- National and Kapodistrian University of Athens (GR)
- Metropolitan Hospital (GR)
Publication Details
- Journal
- Heliyon
- Published
- 2024-09-12
- DOI
- https://doi.org/10.1016/j.heliyon.2024.e37873
- Citations
- 4
- Primary Topic
- Cancer Genomics and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 1.14
Funders
- Stavros Niarchos Foundation
- National and Kapodistrian University of Athens
- National Technical University of Athens