Protocol to generate two distinct standard-of-care murine glioblastoma models for evaluating novel combination therapies
In cancer research, murine models play a crucial role as highly valuable preclinical tools. Here, we present a protocol to generate a murine model of glioblastoma through the direct intracranial injection of tumor cells. We describe steps for cell culture, intracranial implantation, and standard-of-care treatments. We then detail procedures for monitoring tumor growth using bioluminescent imaging. For complete details on the use and execution of this protocol, please refer to Pelizzari-Raymundo et al.<sup>1</sup>.
Authors
- Ulrich Jarry (ORCID: https://orcid.org/0000-0001-7961-0378)
- Diana Pelizzari‐Raymundo (ORCID: https://orcid.org/0000-0002-9071-2380)
- Tony Avril (ORCID: https://orcid.org/0000-0002-1129-8514)
- Éric Chevet (ORCID: https://orcid.org/0000-0001-5855-4522)
- Pierre‐Jean Le Reste (ORCID: https://orcid.org/0000-0003-4673-5697)
- Raphaël Pineau (ORCID: https://orcid.org/0000-0002-0701-7302)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Oncogenesis Stress Signaling (FR)
- Structure Fédérative de Recherche en Biologie et Santé de Rennes (FR)
- Université de Rennes (FR)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-09-01
- DOI
- https://doi.org/10.1016/j.xpro.2024.103304
- Citations
- 2
- Primary Topic
- Glioma Diagnosis and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.88
Funders
- Agence Nationale de la Recherche
- Institut National de la Santé et de la Recherche Médicale
- Ligue Contre le Cancer
- Institut National Du Cancer
- Région Bretagne
- Horizon 2020 Framework Programme