Evaluation of Invitro Anti-diabetic and Anti-oxidant Activity of Stachytarpheta Urticifolia Sims Leaves Extracts

Stachytarpheta urticifolia Sims, “Nettleleaf velvetberry”, belongs to the family Verbena which is a species of lavender plant. It is a perennial herb growing wild and also cultivated as an ornamental weed. The plant was reported to be used in the treatment of various diseases traditionally. This study was held to screen phytochemicals and evaluation of in vitro anti diabetic and antioxidant activity of leaves extracts. The phytochemical analysis revealed the presence of various pharmaceutically active secondary metabolites like alkaloids, saponins, carbohydrate, glycosides, terpenoid, tannins, troterpenoids, polyphenols and flavonoid. On performing extraction with different solvents, ethanol extraction showed a higher percentage yield of 26.63% and followed by the determination of total phenol content showed the value of 254.66 ± 19.55.Based on the phenol content we performed HPTLC analysis of the EESU showed a presence of quercetin with a higher peak of start Rf of 0.398, a max Rf of 0.429 and an end Rf of 0.442.The superoxide radical scavenging activities shows the percentage inhibition of 39.123 to 89.11 at 10 to 50 μg/ml. The hydroxyl and lipid peroxidation activites showed its potential range as a powerful anti-oxidant. α-amylase and α-glucosidase inhibitory activites shows the anti-diabetic activity with IC50 values at the average of 269.88 μg/ml and 351.38 μg/ml. This study conclude valuable insights into the antioxidant, anti-lipid peroxidation, and anti-diabetic properties of Stachytarpheta urticifolia Sims. The results of extract supported the traditional claim of the plant. Therefore, further investigation needs to be performed in vivo anti diabetic activity.