STARDUST: A pipeline for the unbiased analysis of astrocyte regional calcium dynamics
Calcium imaging has become a popular way to probe astrocyte activity, but few techniques holistically capture discrete calcium signals occurring across the astrocyte domain. Here, we introduce STARDUST, a pipeline for the spatio-temporal analysis of regional dynamics and unbiased sorting of transients from fluorescence recordings of astrocytes. We describe steps for installing software, detecting active pixel patches, obtaining region of activity (ROA) maps, and extracting time series from ROAs. We then detail procedures for extracting signal features using custom-made code.
Authors
- Yifan Wu (ORCID: https://orcid.org/0000-0003-0028-8457)
- Katheryn B. Lefton (ORCID: https://orcid.org/0000-0002-1434-7385)
- Thomas Papouin (ORCID: https://orcid.org/0000-0001-5288-1944)
- Timothy E. Holy (ORCID: https://orcid.org/0000-0002-2429-1071)
- Yanchao Dai (ORCID: https://orcid.org/0000-0001-6169-514X)
Institutions
- Washington University in St. Louis (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-09-01
- DOI
- https://doi.org/10.1016/j.xpro.2024.103305
- Citations
- 5
- Primary Topic
- Neuroscience and Neuropharmacology Research
- Type
- article
- Field-Weighted Citation Impact
- 1.34
Funders
- U.S. Department of Defense
- Brain and Behavior Research Foundation
- Whitehall Foundation
- Foundation for Barnes-Jewish Hospital
- Children's Discovery Institute
- National Alliance for Research on Schizophrenia and Depression
- National Institutes of Health
- Center for Cellular Imaging, Washington University
- McDonnell Center for Cellular and Molecular Neurobiology, Washington University in St. Louis
- National Institute of Mental Health