Ganzzellsynthese von Calcidiol mit einem Molybdän-Biokatalysator

Abstract Biotransformations have many advantages over chemical syntheses. By combining highly selective enzymatic reactions with the protective environment of living cells, novel and economically feasible processes are possible. In this article, we present a novel, oxygen-independent whole-cell-based platform for the production of calcidiol, the physiologically active form of vitamin D 3 in the human body. The key biocatalyst involved is a bacterial Mo-dependent hydroxylase, originally discovered in studies of anaerobic cholesterol degradation.