Suitability of undenatured ethanol for DNA and RNA preservation in pig oral fluid and fecal samples used for PCR-based pathogen detection

Nucleic acid integrity in pig oral fluid and fecal samples is important for polymerase chain reaction-based pathogen detection and appropriate preservation during shipping is required. A final concentration of 70% undenatured ethanol was sufficient to maintain DNA and RNA quality for up to 7 days.

Authors

Publication Details

Journal
Journal of Swine Health and Production
Published
2024-09-01
DOI
https://doi.org/10.54846/jshap/1383
Primary Topic
Molecular Biology Techniques and Applications
Type
article
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article

Suitability of undenatured ethanol for DNA and RNA preservation in pig oral fluid and fecal samples used for PCR-based pathogen detection

Joanna Gerszon, Yvette Pollock, Melony J. Sellars, Berit Genz et al.
Journal of Swine Health and Production
Molecular Biology Techniques and Applications
article

Suitability of undenatured ethanol for DNA and RNA preservation in pig oral fluid and fecal samples used for PCR-based pathogen detection

Joanna Gerszon, Yvette Pollock, Melony J. Sellars, Berit Genz, Raif Moser
article en

Abstract

Nucleic acid integrity in pig oral fluid and fecal samples is important for polymerase chain reaction-based pathogen detection and appropriate preservation during shipping is required. A final concentration of 70% undenatured ethanol was sufficient to maintain DNA and RNA quality for up to 7 days.

Journal of Swine Health and ProductionVol. 32(5)
Life below water
Openalex Percentile: Top 86%
Molecular Biology Techniques and Applications
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