Interaction of 5’-monoribonucleotides with Interferon α-2b

Aim: This study aimed to investigate the impact of cytosine 5'-monophosphate (5'-CMP), guanosine 5'-monophosphate (5'-GMP), and uridine 5'-monophosphate (5'-UMP), along with their disodium salts, in combination with D-mannitol (D-M), on the conformation and fluorescence properties of interferon -2b (IFN), and to determine the energy parameters governing these interactions.Methods: Spectroscopic techniques including time-resolved spectroscopy, circular dichroism, and fluorescence spectroscopy, along with isothermal nanocalorimetry, were employed.Results: The nucleotide monophosphates (5'-rN-MFs) in their acidic form, particularly when complexed with D-mannitol, exhibited stronger binding to interferon -2b compared to their salt forms.Interaction between IFN -2b and 5'-rNMFs, especially in the presence of D-mannitol, was found to be exothermic and induced changes in the conformational flexibility of IFN -2b, * The materials of the conference were double-blind peer-reviewed according to the rules of the journal Biopolymers and Cell.