Development of new real-time PCR assays for detection and species differentiation of Plasmodium ovale
We identified promising multi-copy targets for molecular detection and differentiation of P. ovalecurtisi and P. ovalewallikeri and used them to develop real-time PCR assays. The best performing P. ovalecurtisi assay performed well in singleplex and duplex formats, while the P. ovalewallikeri assay did not reliably detect low-density infections in either format. These assays have potential use for high-throughput identification of P. ovalecurtisi, or for identification of higher density P. ovalecurtisi or P. ovalewallikeri infections that are amenable to downstream next-generation sequencing.
Authors
- Rachel Sendor (ORCID: https://orcid.org/0000-0001-7259-1514)
- Jessica T. Lin (ORCID: https://orcid.org/0000-0002-4516-723X)
- Wenqiao He (ORCID: https://orcid.org/0000-0002-0579-6955)
- Antoinette Tshefu (ORCID: https://orcid.org/0000-0001-7657-6901)
- Albert Ntumba Kalonji (ORCID: https://orcid.org/0009-0003-1487-178X)
- Billy Ngasala (ORCID: https://orcid.org/0000-0001-9300-7719)
- Jonathan B. Parr (ORCID: https://orcid.org/0000-0002-4465-9830)
- Varun R. Potlapalli (ORCID: https://orcid.org/0009-0001-0069-1353)
- Jonathan J. Juliano (ORCID: https://orcid.org/0000-0002-0591-0850)
- Fernandine Phanzu
- Kyaw L. Thwai
- Melchior Mwandagalirwa Kashamuka
Institutions
- University of North Carolina at Chapel Hill (US)
- Ministry of Public Health (CD)
- Muhimbili University of Health and Allied Sciences (TZ)
- Sanru (CD)
- Southern Medical University (CN)
Publication Details
- Journal
- PLoS neglected tropical diseases
- Published
- 2024-09-10
- DOI
- https://doi.org/10.1371/journal.pntd.0011759
- Citations
- 2
- Primary Topic
- Malaria Research and Control
- Type
- article
- Field-Weighted Citation Impact
- 1.09
Funders
- Abbott Laboratories
- Global Fund to Fight AIDS, Tuberculosis and Malaria
- Gilead Sciences
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases