Efficacy evaluation of a bivalent subunit vaccine against epidemic PEDV heterologous strains with low cross-protection
The detection rate of PEDV G2a subtype strains is currently increasing. Although commercial vaccines are available, most vaccines do not exert an ideal protective effect against these strains. Furthermore, there is no definitive research into the cross-protection between G2a and G2b strains, and no bivalent vaccine provides joint protection against both. Therefore, in this study, we investigated the cross-protection between PEDV G2a and G2b strains and designed a candidate bivalent subunit vaccine combining the trimeric S proteins of the G2a and G2b subtypes. We demonstrate that the cross-protection between strains G2a and G2b is poor and that this bivalent subunit vaccine protects piglets from viral attack by inducing both active and passive immunity. This study emphasizes the effectiveness of the PEDV G2a/G2b-S bivalent subunit vaccine and provides a feasible method for the development of efficient PEDV vaccines.
Authors
- Jizong Li (ORCID: https://orcid.org/0000-0001-5682-1834)
- Yaoxin Shen (ORCID: https://orcid.org/0000-0002-6848-9258)
- Gege Zhang
- Chuanhong Wang (ORCID: https://orcid.org/0000-0001-8446-9268)
- Baochao Fan (ORCID: https://orcid.org/0000-0001-9780-5080)
- Yunchuan Li (ORCID: https://orcid.org/0000-0002-0612-8924)
- Bin Li (ORCID: https://orcid.org/0000-0003-1318-7081)
- Xuehan Zhang (ORCID: https://orcid.org/0000-0002-0647-2266)
- Mi Hu (ORCID: https://orcid.org/0009-0007-5170-0735)
- Chengcheng Li (ORCID: https://orcid.org/0000-0002-5692-2878)
- Yupeng Li (ORCID: https://orcid.org/0000-0003-1220-4580)
- Shiyu Liu (ORCID: https://orcid.org/0000-0001-8177-3985)
- Min Sun (ORCID: https://orcid.org/0000-0002-9323-0758)
- Shanshan Yang
- Yongxiang Zhao (ORCID: https://orcid.org/0000-0003-3755-0439)
- Rongli Guo
- Xu Song
- Yi-Xiang Wang (ORCID: https://orcid.org/0000-0002-5246-5497)
Institutions
- Hebei Agricultural University (CN)
- Jiangsu University (CN)
- Jiangsu Academy of Agricultural Sciences (CN)
- Yangzhou University (CN)
Publication Details
- Journal
- Journal of Virology
- Published
- 2024-09-10
- DOI
- https://doi.org/10.1128/jvi.01309-24
- Citations
- 30
- Primary Topic
- Animal Virus Infections Studies
- Type
- article
- Field-Weighted Citation Impact
- 10.23
Funders
- Jiangsu Academy of Agricultural Sciences
- Jiangsu Agricultural Science and Technology Innovation Fund
- National Natural Science Foundation of China
- Government of Jiangsu Province
- Natural Science Foundation of Jiangsu Province
- National Key Research and Development Program of China