Stimulated Brillouin scattering flow cytometry
We present the use of stimulated Brillouin scattering spectroscopy to achieve rapid measurements of cell biomechanics in a flow cytometer setup. Specifically, our stimulated Brillouin scattering flow cytometry can acquire at a rate of 200 Hz, with a spectral acquisition time of 5 ms, which marks a 10x improvement compared to previous demonstrations of spontaneous Brillouin scattering flow cytometry. We experimentally validate our stimulated Brillouin scattering flow cytometer by measuring cell populations of normal breast epithelial cells and metastatic breast epithelial cancer cells.
Authors
- Giulia Zanini (ORCID: https://orcid.org/0000-0002-5990-1183)
- Chenchen Handler
- Michele Vítolo (ORCID: https://orcid.org/0000-0001-8055-9645)
- Giuliano Scarcelli (ORCID: https://orcid.org/0000-0002-1736-077X)
- Stuart S. Martin (ORCID: https://orcid.org/0000-0002-4378-4381)
- Jake R. Rosvold (ORCID: https://orcid.org/0000-0002-7541-1600)
- Jiarui Li (ORCID: https://orcid.org/0000-0003-0132-8270)
- Eric Frank
Institutions
- University of Maryland, Baltimore (US)
- University of Maryland, College Park (US)
Publication Details
- Journal
- Biomedical Optics Express
- Published
- 2024-09-09
- DOI
- https://doi.org/10.1364/boe.537602
- Citations
- 1
- Primary Topic
- Microfluidic and Bio-sensing Technologies
- Type
- article
- Field-Weighted Citation Impact
- 0.18
Funders
- National Cancer Institute