Oxidative Status of Peripheral Blood Mononuclear Cells of Stallions of Different Ages

Background: The aging process causes changes in the structure and functioning of body systems, including the immune system. The aim of this study was to assess the oxidative status of peripheral blood mononuclear cells (PBMCs) of young (3-5 years old), full-aged (6-15 years old) and older stallions (16-25 years old). Methods: The level of protein carbonyl derivatives, low and medium molecular weight substances, the activity of superoxide dismutase, catalase, g-glutamyl transpeptidase, acid phosphatase, lactate dehydrogenase were studied in PBMCs of 31 stallions of Arabian breed. Result: An increase in the total content of carbonyl derivatives was revealed in the group of older stallions compared with young (p=0.0030) and full-aged stallions (p=0.0030) due to an increase in the content most of all carbonyl derivatives of a neutral and basic nature. An increase in the level of low and medium molecular weight substances was recorded in the group of older animals compared with young (p=0.0089) and full-aged stallions (p=0.0102). A decrease in g-glutamyl transpeptidase activity was observed in young stallions compared with full-aged (p=0.0026) and older stallions (p=0.0006). The data obtained indicate a violation of the redox balance in aged stallions‘ PBMCs.