Pan-genome analysis reveals novel chromosomal markers for multiplex PCR-based specific detection of Bacillus anthracis
The study identified thirty chromosome-encoded genes specific to B. anthracis, encompassing previously described genes in known lambda prophage regions and nine newly discovered genes from an undefined gene region to the best of our knowledge. Three multiplex PCR assays offer an accurate and reliable alternative method for detecting B. anthracis. Furthermore, these genetic markers have value in anthrax vaccine development, and understanding the pathogenicity of B. anthracis.
Authors
- Hideaki Higashi (ORCID: https://orcid.org/0000-0002-5370-7865)
- Jargalsaikhan Enkhtuya
- Harvey Kamboyi (ORCID: https://orcid.org/0000-0002-3316-7611)
- Tuvshinzaya Zorigt
- Yoshikazu Furuta (ORCID: https://orcid.org/0000-0003-4710-1389)
- Musso Munyeme (ORCID: https://orcid.org/0000-0001-7224-1071)
- Н Энхцэцэг
- Atmika Paudel (ORCID: https://orcid.org/0000-0003-3478-9452)
- Bernard M. Hang’ombe (ORCID: https://orcid.org/0000-0002-4201-6401)
- Misheck Shawa
- Mungunsar Chuluun
- Badgar Battsetseg
- Misa Sugawara
Institutions
- Mongolian University of Life Sciences (MN)
- Hokkaido University (JP)
- Sapporo International University (JP)
- University of Zambia (ZM)
- Genendeavor (United States) (US)
- Hokkai Gakuen University (JP)
Publication Details
- Journal
- BMC Infectious Diseases
- Published
- 2024-09-09
- DOI
- https://doi.org/10.1186/s12879-024-09817-9
- Citations
- 3
- Primary Topic
- Bacillus and Francisella bacterial research
- Type
- article
- Field-Weighted Citation Impact
- 0.70
Funders
- Japan Agency for Medical Research and Development
- Ministry of Education, Culture, Sports, Science and Technology
- Hokkaido University
- National Institutes of Health
- Japan Society for the Promotion of Science
- National Institute of Allergy and Infectious Diseases