Performance evaluation of microbial fuel cells for bioelectricity generation: influence of potential scan-rate and real-time external load

Abstract The electrochemical performance of microbial fuel cells is conventionally assessed through linear sweep voltammetry at predefined potential scan rates. Nevertheless, this approach frequently falls short in representing the long-term behavior of microbial fuel cells under actual external loads, highlighting the need for a standardized evaluation method incorporating both linear sweep voltammetry and external loads. To address this gap, this study evaluates the performance of single-chamber microbial fuel cells under different loads and scan rates. The MFCs were tested with external loads of 1200, 470, and 270 Ω, derived from maximum power points of polarization sweeps at scan rates of 0.1, 0.5, and 1 mV/s at two operational phases. Power estimates at these scan rates were 61.96, 87.88, and 166.68 mW/m 2 at current densities of 116.5, 229.6, and 403 mA/m 2 , respectively. In the initial two hours, average power densities with 1200, 470, and 270 Ω were 73 ± 16.7, 36.3 ± 42, and 88.5 ± 120.1 mW/m 2 , respectively. Over the long term, the fuel cells under constant loading with resistance estimated at 0.1 mV/s showed average power 73.7% and 89.1% higher than those with resistances estimated at 0.5 mV/s and 1 mV/s, respectively, indicating that higher scan rates lead to overestimation of power. Although initially underestimated, the 0.1 mV/s scan rate more accurately reflected the true long-term performance of the fuel cells. This study emphasizes the importance of using appropriate scan rates for linear sweep voltammetry to obtain realistic long-term performance estimates of microbial fuel cells under real-time loads.