Identification of amino acid residue in the <i>Cronobacter sakazakii</i> LamB responsible for the receptor compatibility of polyvalent coliphage CSP1

Polyvalent phages have the advantage of a broader host range, overcoming the limitation of the narrow host range of phages. However, the limited molecular biological understanding on the host bacteria-polyvalent phage interaction hinders its effective application. Here, we revealed that the ability of the polyvalent phage CSP1 to infect <i>Cronobacter sakazakii</i> ATCC 29544 is disturbed by a single proline residue in the LamB protein and that lipopolysaccharide is used as an auxiliary receptor for CSP1 to support the adsorption and the subsequent infection of <i>C. sakazakii</i>. These results can contribute to a better understanding of the interaction between polyvalent phages and host bacteria for efficient phage application.