Rapid real-time quantitative colorimetric LAMP methodology for field detection of Verticillium dahliae in crude olive-plant samples
This study describes the development of a new real time LAMP assay, targeting the ITS region of the rRNA gene of V. dahliae in olive trees combined with a 3D-printed portable device for field testing using a tablet. The assay is characterized by high sensitivity and specificity as well as ability to operate using directly crude samples such as woody tissue or petioles. The reported methodology is setting the basis for the development of an on-site detection methodology for V. dahliae in olive trees, but also for other plant pathogens.
Authors
- George Papadakis (ORCID: https://orcid.org/0000-0001-5964-6568)
- Aliki K. Tzima (ORCID: https://orcid.org/0000-0002-1178-8222)
- Maria Megariti
- Epaminondas J. Paplomatas (ORCID: https://orcid.org/0000-0002-2929-0676)
- Alexandros Pantazis (ORCID: https://orcid.org/0000-0002-6774-4173)
- Electra Gizeli (ORCID: https://orcid.org/0000-0002-3598-5984)
- Emmanouil A. Markakis (ORCID: https://orcid.org/0000-0002-8791-0669)
- Alexandra Panagou
- Georgios Patsis
Institutions
- Agricultural University of Athens (GR)
- Mediterranean University (ME)
- Hellenic Mediterranean University (GR)
- FORTH Institute of Molecular Biology and Biotechnology (GR)
- Institute of Molecular Biology and Biotechnology (BG)
- Foundation for Research and Technology Hellas (GR)
Publication Details
- Journal
- Plant Methods
- Published
- 2024-09-09
- DOI
- https://doi.org/10.1186/s13007-024-01251-x
- Citations
- 5
- Primary Topic
- Biosensors and Analytical Detection
- Type
- article
- Field-Weighted Citation Impact
- 0.92
Funders
- General Secretariat for Research and Technology