<i>BonnMu</i>: A Resource for Functional Genomics in Maize (<i>Zea mays</i> L.)
The <i>BonnMu</i> resource is a public transposon-tagged population designed for reverse and forward genetics studies in maize (<i>Zea mays</i> L.). The resource was created by crossing an active <i>Mutator</i> (<i>Mu</i>) transposon line into different inbred lines to induce insertional mutations. The resulting F<sub>1</sub> generation was self-pollinated to generate segregating <i>BonnMu</i> F<sub>2</sub> stocks. The <i>Mu</i>-tagged <i>BonnMu</i> F<sub>2</sub> stocks have insertions in 83% of all annotated maize gene models, and <i>Mu</i> insertion positions and photos of the seedling phenotypes of the segregating <i>BonnMu</i> F<sub>2</sub> stocks are deposited in the Maize Genetics and Genomics Database (MaizeGDB), with seeds available to the community. Here, we discuss the creation, expansion, and application of the <i>BonnMu</i> resource for identifying and characterizing mutations induced by <i>Mu</i> transposons, which represents a useful tool for functional genomics studies in maize.
Authors
- Xuelian Du (ORCID: https://orcid.org/0000-0002-4611-7453)
- Frank Hochholdinger (ORCID: https://orcid.org/0000-0002-5155-0884)
- Yan Naing Win (ORCID: https://orcid.org/0000-0002-4043-5194)
- Caroline Marcon (ORCID: https://orcid.org/0000-0001-6699-9441)
Institutions
- University of Bonn (DE)
Publication Details
- Journal
- Cold Spring Harbor Protocols
- Published
- 2024-09-09
- DOI
- https://doi.org/10.1101/pdb.top108465
- Citations
- 3
- Primary Topic
- Chromosomal and Genetic Variations
- Type
- article