<i>BonnMu</i>: A Resource for Functional Genomics in Maize (<i>Zea mays</i> L.)

The <i>BonnMu</i> resource is a public transposon-tagged population designed for reverse and forward genetics studies in maize (<i>Zea mays</i> L.). The resource was created by crossing an active <i>Mutator</i> (<i>Mu</i>) transposon line into different inbred lines to induce insertional mutations. The resulting F<sub>1</sub> generation was self-pollinated to generate segregating <i>BonnMu</i> F<sub>2</sub> stocks. The <i>Mu</i>-tagged <i>BonnMu</i> F<sub>2</sub> stocks have insertions in 83% of all annotated maize gene models, and <i>Mu</i> insertion positions and photos of the seedling phenotypes of the segregating <i>BonnMu</i> F<sub>2</sub> stocks are deposited in the Maize Genetics and Genomics Database (MaizeGDB), with seeds available to the community. Here, we discuss the creation, expansion, and application of the <i>BonnMu</i> resource for identifying and characterizing mutations induced by <i>Mu</i> transposons, which represents a useful tool for functional genomics studies in maize.

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Publication Details

Journal
Cold Spring Harbor Protocols
Published
2024-09-09
DOI
https://doi.org/10.1101/pdb.top108465
Citations
3
Primary Topic
Chromosomal and Genetic Variations
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article
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article

BonnMu: A Resource for Functional Genomics in Maize (Zea mays L.)

Xuelian Du, Frank Hochholdinger, Yan Naing Win, Caroline Marcon
3 citations
Cold Spring Harbor Protocols
Chromosomal and Genetic Variations
article

BonnMu: A Resource for Functional Genomics in Maize (Zea mays L.)

Xuelian Du, Frank Hochholdinger, Yan Naing Win, Caroline Marcon
article en
3 citations

Abstract

The <i>BonnMu</i> resource is a public transposon-tagged population designed for reverse and forward genetics studies in maize (<i>Zea mays</i> L.). The resource was created by crossing an active <i>Mutator</i> (<i>Mu</i>) transposon line into different inbred lines to induce insertional mutations. The resulting F<sub>1</sub> generation was self-pollinated to generate segregating <i>BonnMu</i> F<sub>2</sub> stocks. The <i>Mu</i>-tagged <i>BonnMu</i> F<sub>2</sub> stocks have insertions in 83% of all annotated maize gene models, and <i>Mu</i> insertion positions and photos of the seedling phenotypes of the segregating <i>BonnMu</i> F<sub>2</sub> stocks are deposited in the Maize Genetics and Genomics Database (MaizeGDB), with seeds available to the community. Here, we discuss the creation, expansion, and application of the <i>BonnMu</i> resource for identifying and characterizing mutations induced by <i>Mu</i> transposons, which represents a useful tool for functional genomics studies in maize.

Cold Spring Harbor ProtocolsVol. 2025(9)
University of Bonn (DE)
Chromosomal and Genetic Variations
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<i>BonnMu</i>: A Resource for Functional Genomics in Maize (<i>Zea mays</i> L.) — Xuelian Du, Frank Hochholdinger, et al. · Cold Spring Harbor Protocols (2024) | TGRS Research Map | TGRS