NLRP1-dependent activation of Gasdermin D in neutrophils controls cutaneous leishmaniasis
Intracellular pathogens that replicate in host myeloid cells have devised ways to inhibit the cell's killing machinery. Pyroptosis is one of the host strategies used to reduce the pathogen replicating niche and thereby control its expansion. The intracellular Leishmania parasites can survive and use neutrophils as a silent entry niche, favoring subsequent parasite dissemination into the host. Here, we show that Leishmania mexicana induces NLRP1- and caspase-1-dependent Gasdermin D (GSDMD)-mediated pyroptosis in neutrophils, a process critical to control the parasite-induced pathology. In the absence of GSDMD, we observe an increased number of infected dermal neutrophils two days post-infection. Using adoptive neutrophil transfer in neutropenic mice, we show that pyroptosis contributes to the regulation of the neutrophil niche early after infection. The critical role of neutrophil pyroptosis and its positive influence on the regulation of the disease outcome was further demonstrated following infection of mice with neutrophil-specific deletion of GSDMD. Thus, our study establishes neutrophil pyroptosis as a critical regulator of leishmaniasis pathology.
Authors
- Borja Prat-Luri (ORCID: https://orcid.org/0000-0002-6120-1370)
- Benjamin Demarco (ORCID: https://orcid.org/0000-0002-5537-1381)
- Benjamin P. Hurrell (ORCID: https://orcid.org/0000-0001-7753-2927)
- Petr Brož (ORCID: https://orcid.org/0000-0002-2334-7790)
- Oaklyne Billion
- Katiuska Passelli (ORCID: https://orcid.org/0000-0002-6785-1323)
- Michiel Goris (ORCID: https://orcid.org/0000-0002-3969-7162)
- Bernard Malissen
- Míriam Díaz-Varela (ORCID: https://orcid.org/0000-0002-8753-8115)
- Chantal Desponds
- Sanam Peyvandi (ORCID: https://orcid.org/0000-0002-3231-1116)
- Shaden Kamhawi (ORCID: https://orcid.org/0000-0003-4304-636X)
- Fabienne Tacchini‐Cottier (ORCID: https://orcid.org/0000-0003-3561-0207)
- Somaditya Dey (ORCID: https://orcid.org/0000-0002-5741-9668)
- Eva Iniguez (ORCID: https://orcid.org/0009-0006-9027-0523)
- Manon Termote
Institutions
- University of Southern California (US)
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Institute for Work and Health (CH)
- Government of West Bengal (IN)
- National Institute of Allergy and Infectious Diseases (US)
- Centre d’Immunologie de Marseille-Luminy (FR)
- Vector Oncology (United States) (US)
- Centre d'Immunophénomique
- West Bengal State University (IN)
- University of Lausanne (CH)
Publication Details
- Journal
- PLoS Pathogens
- Published
- 2024-09-09
- DOI
- https://doi.org/10.1371/journal.ppat.1012527
- Citations
- 4
- Primary Topic
- Research on Leishmaniasis Studies
- Type
- article
- Field-Weighted Citation Impact
- 2.17
Funders
- National Science Foundation
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases
- Indian Council of Medical Research
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Albert-Ludwigs-Universität Freiburg
- Université de Lausanne
- National Institutes of Health