Primed and ready: nanopore metabarcoding can now recover highly accurate consensus barcodes that are generally indel-free
Our results demonstrate that nanopore sequencing can generate metabarcodes with Illumina-like accuracy, and we are the first study to show that nanopore metabarcodes are almost always indel-free. We also show that nanopore metabarcoding is viable for characterising species-rich communities rapidly, and that the same ecological conclusions can be obtained regardless of the sequencing platform used. Collectively, our study inspires confidence in nanopore sequencing and paves the way for greater utilisation of nanopore technology in various metabarcoding applications.
Authors
- Danwei Huang (ORCID: https://orcid.org/0000-0003-3365-5583)
- Jia Jin Marc Chang (ORCID: https://orcid.org/0000-0001-5208-4929)
- Maxine A. D. Mowe (ORCID: https://orcid.org/0000-0001-9060-4163)
- Yin Cheong Aden Ip (ORCID: https://orcid.org/0000-0002-9472-1161)
- Zeehan Jaafar (ORCID: https://orcid.org/0000-0002-7311-7491)
- Wan Lin Neo
Institutions
- National University of Singapore (SG)
- University of Washington (US)
Publication Details
- Journal
- BMC Genomics
- Published
- 2024-09-09
- DOI
- https://doi.org/10.1186/s12864-024-10767-4
- Citations
- 17
- Primary Topic
- Environmental DNA in Biodiversity Studies
- Type
- article
- Field-Weighted Citation Impact
- 7.80
Funders
- National Research Foundation
- National Research Foundation Singapore
- National Parks Board - Singapore
- National Supercomputing Centre Singapore